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KMID : 0379520130290040293
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한국독성학회지
2013 Volume.29 No. 4 p.293 ~ p.298
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Real-time Assay of Toxic Lead in In Vivo Living Plant Tissue
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Ly Suw-Young
Kim Nack-Joo
Youn Min-sang
Kim Yong-wook
Sung Yeol-min
Kim Do-hoon
Chung Tack-hyun
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Abstract
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A method of detecting lead was developed using square wave anodic stripping voltammetry (SWASV) with DNA-carbon nanotube paste electrode (CNTPE). The results indicated a sensitive oxidation peak current of lead on the DNA-CNTPE. The curves were obtained within a concentration range of 50 ngL?1-20 mgL?1 with preconcentration time of 100, 200, and 400 sec at the concentration of mgL?1, μgL?1, and ngL?1, respectively. The observed relative standard deviation was 0.101% (n = 12) in the lead concentration of 30.0 μgL?1 under optimum conditions. The low detection limit (S/N) was pegged at 8 ngL?1 (2.6 × 10?8 M). Results showed that the developed method can be used in real-time assay in vivo without requiring any pretreatment and pharmaceutical samples, and food samples, as well as other materials requiring water source contamination analyses.
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KEYWORD
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DNA-CNTPE, Lead anodic stripping voltammetry, Low concentration, In vivo, Plant, Tap water
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